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GEL electrophoresis
Gel Electrophoresis Gel electrophoresis is a method by which DNA fragments or other macromolecules (RNA, proteins, etc.,) are dissociated according to their varying size and charge. DNA samples are loaded into small indentations known as 'wells' which are arranged along the extreme side of the gel.Read more
Gel Electrophoresis
Gel electrophoresis is a method by which DNA fragments or other macromolecules (RNA, proteins, etc.,) are dissociated according to their varying size and charge. DNA samples are loaded into small indentations known as ‘wells’ which are arranged along the extreme side of the gel. The gel molded like a slab, is made from a polysaccharide called ‘agarose’ and is highly porous in nature.
When electric current is passed along this apparatus, the DNA will move towards the positive electrode in virtue of its negative charge. Small fragments of DNA move swiftly compared to the larger ones. After the partition the DNA fragments can be visualized as bands of different sizes by utilizing DNA binding dyes an UV light.
This method has widespread practical applications in Polymerase Chain Reaction, DNA Finger Printing, detection of specific gene sets and species identification in molecular levels.
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