What is the use of Likers and Adapters in rDNA technology?
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Linkers and adaptors play crucial role in the construction and manipulation of recombinant DNA molecules.
Linkers are short , synthetic, double stranded DNA sequences that containd on or more restriction enzyme recognition site.The major role of linkers are facilitating ligation, adding restriction site, creating sticky ends. Linkers when added to the blunt ends of the DNA fragments convert them into the sticky ends.
Adaptors are short, double stranded DNA sequences with one sticky end and blunt end. Unlike linkers, adaptors are pre cut to have sticky ends that matches the restriction sites of the target vector. The major role of adaptors is to convert blunt ends into sticky ends, preventing self ligation, and facilitating ligation.
Both linkera and adaptors are essential tool in molecular cloning , enabling the precise manipulation of DNA sequences for construction of recombinant DNA molecules.
Why Cosmid is considered more effective that plasmid?
Cosmids are considered more effective than plasmids in certain application of genetic engineering and molecular cloning.
Cosmids can carry larger DNA inserts ,typically 35 to 45 kilobases, compared to plasmids which has smaller cloning capacity,usually upto 10 to 15 kilo bases.
Cosmids are hybrid vectors that combines features of plasmids and bacteriophages while plasmids are simple circular DNA molecule that replicates independently within the host cell.
Linkers and adaptors play crucial role in the construction and manipulation of recombinant DNA molecules.
Linkers are short , synthetic, double stranded DNA sequences that containd on or more restriction enzyme recognition site.The major role of linkers are facilitating ligation, adding restriction site, creating sticky ends. Linkers when added to the blunt ends of the DNA fragments convert them into the sticky ends.
Adaptors are short, double stranded DNA sequences with one sticky end and blunt end. Unlike linkers, adaptors are pre cut to have sticky ends that matches the restriction sites of the target vector. The major role of adaptors is to convert blunt ends into sticky ends, preventing self ligation, and facilitating ligation.
Both linkers and adaptors are essential tool in molecular cloning , enabling the precise manipulation of DNA sequences for construction of recombinant DNA molecules.