what are the procedure of isolating any heavy metal resistant gene from Bacillus species ?
India played an important role in the Cold War, even though it wasn't directly part of either the US-led or Soviet-led groups. Here's a simple explanation of India's involvement: India became independent in 1947, right when the Cold War was starting. Its leader, Jawaharlal Nehru, decided India shoulRead more
India played an important role in the Cold War, even though it wasn’t directly part of either the US-led or Soviet-led groups. Here’s a simple explanation of India’s involvement:
India became independent in 1947, right when the Cold War was starting. Its leader, Jawaharlal Nehru, decided India should stay neutral. This idea became known as “non-alignment.” It meant India wouldn’t fully support either the US or the Soviet Union.
However, India still had relationships with both sides:
1. India got economic help and some military equipment from the Soviet Union.
2. It also received aid from the US, especially food.
India tried to stop the spread of communism in Asia but didn’t want to join US military alliances. This sometimes caused tension with the US.
India also played a role in ending some Cold War conflicts. For example, it helped negotiate peace in Korea in the 1950s.
In the 1960s and 1970s, India moved closer to the Soviet Union. This was partly because the US was friendly with India’s rival, Pakistan. India and the Soviet Union signed a friendship treaty in 1971.
Despite this closeness to the Soviets, India kept its policy of non-alignment. It didn’t become a Soviet ally like some other countries did.
India’s position helped create the Non-Aligned Movement. This was a group of countries that didn’t want to fully support either the US or Soviet side in the Cold War.
By staying neutral, India was able to get benefits from both sides without getting directly involved in their conflicts. This approach helped shape India’s foreign policy even after the Cold War ended.
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To isolate heavy metal resistant genes from Bacillus species, start by collecting environmental samples. Next, isolate Bacillus species using selective media and confirm their presence through tests. Then, extract DNA, amplify the genes using PCR, and confirm amplification through gel electroRead more
To isolate heavy metal resistant genes from Bacillus species, start by collecting environmental samples. Next, isolate Bacillus species using selective media and confirm their presence through tests. Then, extract DNA, amplify the genes using PCR, and confirm amplification through gel electrophoresis. Clone the gene into a vector, like a plasmid, and transform it into bacterial cells for selection. Screening helps identify cells with the gene. Further analysis, such as DNA sequencing, ensures the gene’s presence and integrity in the cloned plasmid. The process may vary based on the specific gene and research protocol. Attention to detail is crucial for accurate results.
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