To isolate heavy metal resistant genes from Bacillus species, start by collecting environmental samples. Next, isolate Bacillus species using selective media and confirm their presence through tests. Then, extract DNA, amplify the genes using PCR, and confirm amplification through gel electrophoresis. Clone the gene into a vector, like a plasmid, and transform it into bacterial cells for selection. Screening helps identify cells with the gene. Further analysis, such as DNA sequencing, ensures the gene’s presence and integrity in the cloned plasmid. The process may vary based on the specific gene and research protocol. Attention to detail is crucial for accurate results.